Case 1443450 DNA Analysis Report Complaint 9944?43984 Investigating Officer: Offense: Assault on LEO Department Name: Saint Louis County P. D. P.O. Wilson SUSPECT: Michael Brown Evidence submitted for DNA analysis (Note: RSS indicates reddish-brown stain): Q2-1 Q5 Q6 QT QB Q94 IQ11 Q12 Q13 Q14 Q15 Q16 Q18 Q19 (120-1 K1 K2 Swabs from Michael Brown?s t?shirt (shirt, item Swabs from Michael Brown's shorts (shorts, item Swabs'from the palm of Michael Brown's left hand (RES) (item Swabs from the back of Michael Brown's left hand (RBS) (item Swabs from the palm of Michael Brown's right hand (RBS) (item Swabs from the back of Michael Brown's right hand (RBS) (item #10) Swab from the ?ngernail of Michael Brown?s left hand item #11) Swab from the fingernail of Michael Brown's right hand (RSS) item #12) Piece of apparent tissue or hardened nasal mucus from the driver front exterior door of Ferguson PD. vehicle 108 (RSS) (item Swab from the driver rear passenger exterior door of Ferguson P.D. vehicle 108 (RES) (item Swab from roadway in front of 2943 Can?eid (RBS) (item #19) Swab from roadwayr in front of 2943 Can?eld (RBS) (item #20) Swabs from PBS on the upper left thigh of PO. Wilson's uniform pants (item #213?1) Swabs from top exterior left front door of Ferguson PD. vehicle 103 (item Swabs from exterior left front door mirror of Ferguson PD. vehicle 108 (item Swabs from interior left front door handle of Ferguson P.D. vehicle 108 (RSS) (item Swabs from PD. Wilson's (RSS) (item #18) Swabs from P.O. Wilson?s uniform shirt left side and collar (shirt, item #2251) Swabs from Wilson?s uniform pants left side (pants, item #29) Buccal swab reference sample from PD. Wilson Bloodstain card reference sample from Michael Brown DNA Case #14-o450 August 14, 2014 Parts: 1 of 3 ext DNA EXAMINATION Amplification of extracted DNA from the above evidence was performed using the Polymerase Chain Reaction (PCR) and profiled at the following STR loci (locus abbreviations used in this report are noted in parenthesis}: D381358 (DB), D168539 (D16), CSF1PO (CSF), TPOX, D881179 (D8), D21311 (D21), (D18). D23441, Digs-433 (D19), THU1, FGA, D5831B (D5), D13331T (D13), D78820 (D7), SE33. [31081248 (D10), (D1), D123391 (D12), D231338, DY3391 (STR IDGUS on the male chromosome), indel (a insertionlcleletion locus), and the sex-determining marker, Amelogenin. RESULTS OF AND CONCLUSION The DNA typing results obtained from (Michael Brown?s t?shirt) are consistent with being a mixture of two or more individuals. This pro?le can be separated into a major component pro?le consistent with Michael Brown and one or. more trace contributors. Due to the limited genetic information available from the trace component of the mixture, no inciusionaryr statements will be made regarding the trace component. Wilson is excluded as a contributor to this mixture pro?le. The DNA typing results obtained from 02-1 (Michael Brown's shorts) are consistent with being a mixture of three or more individuals. This pro?le can be separated into a major component pro?le consistent with Michael Brown and two or more trace contributors. Due to the limited genetic information available from the trace component of the mixture, no inclusionary statements will be made regarding the trace component. P.O. Wilson is excluded as a contributor to this mixture pro?le. The DNA typing results obtained from 05 (palm of Michael Brown's left hand, R83) are consistent with being a mixture of two or more individuals. This pro?le can be separated into a major component profile consistent with Michael Brown and a minor contributor. One additional allele indicates a possible trace contributor. Due to the limited genetic information available from the possible trace contributor. no conclusions regarding the possible trace contributor can be made. PD. Wilson is included as a possible source of the minor contributor pro?le. The observed mixture pro?le is 98 times more liker if it originated from Michael Brown and PD. Wilson than if it originated from Michael Brown and an unknown individual in the in the general unrelated population. The male DNA pro?le obtained from 06 (backof Michael Brown's left hand, RES), (palm of Michael Brown?s right hand, R38), and 08 (back of Michael Brown's right hand, R33) is consistent with the DNA pro?le of Michael Brown. The DNA typing results obtained from 09-1 (Michael Brown?s left hand fingernail are consistent with being a mixture of three or more individuals. This profile can be separated into a major component profile consistent with Michael Brown and two or more trace contributors. Due to the limited genetic information available from the trace component of the mixture, no inclusionary statements will be made regarding the trace component. PD. Wilson is excluded as a contributor to this mixture pro?le. The male DNA pro?le obtained from 010-1 (Michael Brown's right hand fingernail scrapingsi?clippings, R83) is consistent with the DNA profile of Michael Brown. Michael Brown is the source* of the (DNA pro?le obtained from Q11 (apparent tissue or hardened nasal mucus from vehicle 108), the 012 (driver rear passenger exterior door of Ferguson PD. vehicle 108, RES), 013 (R88 from roadway in front of 2943 Can?eld), and 014 (R83 from roadway in front of 2943 Canfield). The DNA typing results obtained from 015 (R83 on the upper left thigh of PD. Wilson's uniform pants) are consistent with being a mixture of two or more individuals. This profile can be separated into a major male component profile and a minor contributor consistent with PD. Wilson. One additional allele indicates a possible trace contributor. Due to the limited genetic information available from the possible trace contributor, no conclusions regarding the possible trace contributor can be made. Michael Brown is the source* of the major male component pro?le detected from Of 5. The DNA typing results obtained from Q16 (top exterior left front door of vehicle 108) are consistent with being a mixture of three or more individuals. This pro?le can be separated into a major mixture of two individuals with a trace contributor. Due to the limited genetic information available from the trace contributor, no conclusions regarding the DNA Case #1443450 August 14, 2014 Page 2 of 3 H. it" possible trace contributor can be made. Michael Brown and PD. Wilson are included as contributors to this major mixture pro?le. The observedlmajor mixture profile is 6.9 million times more likely if it originated from Michael Brown and PD. Wilson than if it originated from P.O. Wilson and an unknown individLial in the in the general unrelated population. The partial DNA pro?le obtained from (2117 (exterior left front door mirror of vehicie 108) is consistent with being a mixture of two or more individuals. Due to the limited genetic information avaiiable the presence or absence of PO. Wilson and Michael Brown cannot be determined. The DNA typing results obtained from 013 (interior left front door handle of vehicle 108) are consistent with being a mixture of two or more individuals. This pro?le can be separated into a major male contributor pro?le and one or more minor contributors. Michaei Brown is the source" of the major male contributor profile. Due to the limited genetic information available from the minor component the presence or absence of PD. Wilson cannot be determined. The DNA typing results'obtained from 019 (P.O.7Wilson?s R85) are consistent with being a mixture of three or more individuals. This profile can be separated into a major mixture of two individuals with a trace contributor. Due to the limited genetic information available from the trace contributor, no conciusions regarding the possible trace contributor can be made. Michael Brown and PD. Wilson are included as contributors to this major - mixture pro?le. The observed major mixture pro?le is 2.1 octillion times more likely if it originated from Michael Brown and P.D. Wilson than if it originated from PD. Wilson and an unknown individual in the in the general unrelated population. The DNA typing results obtained from 020-1 (RD. Wilson?s uniform shirt left side and collar) are consistent with being a mixture of three or more individuals. This profile can be separated into a major mixture of two individuals with a trace component. Due to the limited genetic information avaiiable from the trace component, no conclusions regarding the possible trace contributor canoe made. Michael Brown and PD. Wilson are included as contributors to this major mixture pro?le. The observed major mixture pro?le is 2.1 trillion times more likely if it originated from Michael Brown and P.O. Wilson than if it originated from PD. Wilson and an unknown individual in the in the general unrelated population. The DNA typing results obtained from 021-1 (PD. Wilson?s uniform pants left side) are consistent with being a mixture of three or more individuals. This pro?le can be separated into a major mixture of two individuals with a trace component. Due to the limited genetic information available from the trace component, no conclusions regarding the possible trace contributor can be made. Michaei Brown and PD. Wilson are included as contributors to this major mixture pro?le. The observed major mixture profits is 34 sextillion times more likely if it originated from Michael Brown and PD. Wilson than if it originated from RD. Wilson and an unknown individual in the in the general unrelated population. *Conciusion based on the calculated frequency of the DNA pro?le being rarer than approximately 1 in 310 billion unrelated individuals (Caucasian, African American, Hispanic, and Asian population groups) which is approximately one thousand times the popuiation of the United States. identicai siblings will share the same DNA pro?le. The DNA typing results obtained from K2 and (112 will be transferred to CODIS (Combined DNA Index System) and searched against other DNA pro?les on a regular basis. Should a match be generated, a notification will be sent. PRESERVATION OF SAMPLES The remaining portion(s) of the above evidence and a portion of any remaining extracted DNA that may still exist are being preserved in the St. Louis County Police Crime Laboratory for any additionai tests that may be requested in the future. Me 7 Technical Reviewer, DSN, Date DNA Technical Leader DNA Case #14-D45l} August 14, 2014 Page 3 of 3 DNA Analysis Report St. Louis County Police Department Accredited since 2005 DNA Case it 14-D450-2 Complaint 994 4?43984 Investigating Officer: Offense: Assault on LEO Department Name: Saint Louis County P. D. 7 VICTEM: P.O. Wilson SUSPECT: Michael Brown This report is a supplement to the original report 14-D450 dated August 14, 2014. Evidence submitted for DNA analysis (Note: RBS indicates reddish-brown stain): Q22-1 Swabs from red "Cardinals" baseball cap (item - Q23-1 Swab from RBS on Left ?Nike? sandal (item (323?2 Swabs from Left ?Nike? sandal (item 024-1 Swab from RBS on Right "Nike" sandal (item 024-2 Swabs from Right "Nike" sandal (item (125-1 Swabs from black, yellow, and white rubber bracelet (item (128-1 Swabs from dark brown beaded bracelet (item Evidence previously submitted (refer to original report 14-0450): K1 Buccai swab reference sample from P. O. Wilson K2 Bloodsrain card reference sample from Michael Brown DNA EXAMINATION Amplification of extracted DNA from the above evidence was performed using the Polymerase Chain Reaction (PCR) and profiled at the foflowing STR loci (locus abbreviations used in this report are noted in parenthesis): D381358 (D3), vW D168539 (D16), TPOX, D8811i?9 (D8), D21S11 (D21), D18851 (D18), D23441, D1984 (D19), THD1, FGA, D5SS1B (D5). D138317 (D13), SE33, D1OS1248 (D10), D181656 (D1), D123391 (D12), D281338, (STR locus on the maie chromosome), indel (a insertionideletion locus), and the sex?determining marker, Ameiogenin. RESULTS OF ANALYSIS AND The DNA typing results obtained from 022-1 (?Cardinals? baseball cap) are consistent with being a mixture of two or more individuals. This pro?le can be separated into a major component pro?le and one or more trace contributors. Due to the limited genetic information available from the trace component of the mixture, no inclusionary statements will be made regarding the trace component. The presence or absence of PD. Wilson as a possible trace contributor cannot be determined. Michael Brown is the source* of the major male component profile detected from 022-1. Michael Brown is the source" of the DNA profile obtained from 023-1 (RBS on Left "Nike" sandal) and 024-1 (RBS on Right ?Nike? sandal). DNA Case August 21, 2014 Page 1 of 2 No definitive DNA typing results were obtained from 023-2 (Left "Nike" sandal) and 024-2 (Right "Nike" sandal). Due to the limited genetic information obtained, no conclusions regarding these samples wiil be made. The DNA typing resolts obtained from 025-1 (black, yellow, and white rubber bracelet) are consistent with being a mixture of two or more individuals. This pro?le can be separated into a major male component pro?le and one or more minor contributors. Due to the limited genetic information available from the minor component of the mixture, no inclusionary statements will be, made regarding the minor component. Michael Brown and PD. Wilson are excluded as contributors of the major male component profile, however, the presence or absence of Michael Brown and PO. Wilson as possible minor contributors cannot be determined. The DNA typing results obtained from (126-1 (dark brown beaded bracelet) are consistent with being a mixture of three or more individuals. This profile can be separated into a major male component profile and two or more minor contributors. Due to the limited genetic information avaiiabie from the minor component of the mixture, no inclusionary statements will be made regarding the minor component. Michael Brown and PO. Wilson are excluded as contributors of the major male component profile, however, the presence or absence of Michael Brown and PO. Wilson as possible minor contributors cannot be determined. *Conclusion based on the calcuiated frequency of the DNA profile being rarer than approximately 1 in 310 biliion unrelated individuals (Caucasian, African American, Hispanic, and Asian population groups) which is approximately one thousand times the population of the United States. identical siblings will share the same DNA pro?le. No DNA pro?le will be transferred to (Combined DNA Index System}. PRESERVATION OF SAMPLES The remaining portion(s) of the above evidence and a portion of any remaining extracted DNA that may still exist are being preserved in the St. Louis County Police Crime Laboratory for any additional tests that may be requested in the future. lechnical Reviewer, DSN, Date DNA Technical Leader DNA Case #14-0450-2 August 21, 2014 Pace 2 of 2 7900 Blvd. Saint Louis, MO 63105 Education 05/09 Emmi: Master of Arts - Biology Washington University in St. Louis 05f04 Bachelor of Science Biology, minor in Chemistry Bachelor of Arts Anthropology Departmental Honors (Biology) University of Missouri-Columbia Experience 211 l-present Forensic DNA Technical Leader, St. Louis County Police Department Crime Laboratory II Manage the technical operations of the Laboratory I Ensure compliance with all FBI and ASCLD accreditation standards for forensic DNA analysis I Perform and approve validation studies to bene?t the Laboratory 0 Coordinate and oversee technical problem solving of analytical methods, oversee training, quality assurance, and pro?ciency testing as it pertains to the DNA Unit and review all above documents on an annual basis i Perform DNA casework and casework review; prepare reports and 12105-2/1 1 if05-12f05 testify to results in court Criminalist - DNA Analyst and back-up CODIS Administrator (6f10 to It'll), St. Louis Metropolitan Police Department Crime Laboratory Assist the CODIS Administrator with CODIS hit dispositions, noti?cations, and record keeping Purify, quantitate and amplify forensic samples using robots, ABI 7500 (Quanti?ler and Quanti?ler Duo kits) and ldenti?ler Perform STR DNA typing on forensic samples using 3108} 30 Genetic Analyzers and GeneMapper ID software Prepare reports and testify to results in court Train new in forensic biology and DNA analysis Perform validation studies to bene?t the Laboratory Contract Forensic Biologist, St. Louis Metropolitan Police Department Crime Laboratory Updated 10/7/14 Screen evidence for biological ?uids using an alternate light source, STMP, and other presumptive tests Take cuttings or swabs of biological materials and retain for DNA Page 1 of?if analysis - I Examine slides using oil immersion microscopy I Prepare reports based on laboratory results using JusticeTrax- LIMS softWare Intern at St. Charles County Crime Laboratory, St. Charles Missouri I Participated in validations of ABAcard, HemaTrace and Phadebas I Observed working of rape, homicide, and drug cases including use of TIR, and presumptive color tests MOI-6104 Student Assistant at Research Reactor, University of Missouri (UMC) I Trained employees in laboratory procedures and safety policies I Revised safety procedures and protocol for laboratory equipment I Prepared standards and archaeological samples for Neutron Activation Analysis (N AA) I Utilized Spectrometry, Scanning Electron Microscope, analytical balances, fume hoods, ovens and gas torches I Worked with hazardous materials including radioactive material, Nitric, Hydrochloric and Hydro?uoric acids weekly 6f02-8f02 Undergraduate Researcher at Research Reactor, UMC Funded by Life Sciences Undergraduate Research Opportunity Program I Investigated Bariumetrontium ratios in prehistoric human bones using Laser Ablation to determine dietary patterns Association Memberships 02X 10 American Academy of Forensic Sciences Member in Criminalistics, 2014 Midwestern Association of Forensic Scientists Regular Member, 912012 Additional TraininglMeetings 09/14 ASCLDILAB International Internal Auditor Training Course Sponsored by the St. Louis County Police Academy Instructor: Emma Dutton 082? 14 Association of Forensic DNA and Administrators Summer 2014 Sponsored by AFDAA Houston Texas Instructors: various speakers 07/ 14 Future Trends in Forensic DNA Technology Seminar San Francisco Sponsored by Life Technologieszhermo Scienti?c Instructors: various speakers 041?14 MiduAmerica 2014 Forensic DNA Conference Sponsored by Paternity Testing Corporation Updated 10/73/14 Page 2 of 7 08113 05:13 04MB 03f13 01f13 04KB 02(12 Workshop: ISO 17025 Top Ten Non?Conformances Workshop: ISO 17025 Root Cause Analysis Instructor for workshops: Anna Yoder Grant Writing USA Workshop Sponsored by the St. Louis County Police Academy Instructor: Jennene Colky 40lth Annual Symposia Workshops: Improving the Effectiveness of Forensic Services: Using the FDRESIGHT Project Instructors: Paul J. Speaker and Richard Riley Laboratory Lean Six Sigma: A Recipe for Dramatic Ef?ciency and Quality Instructors: Tim Adam Becnel, and Cami Green Using FORESIGHT Output to Improve Employee Retention, Satisfaction, and Leverage More Resources Instructors: David Dawiey, Richard Riley and Paul Speaker DNA Analyst Training on Mixture Interpretation Sponsored by NIST (vveb delivered) Instructors: John Butler, Bruce Heidebrecht, Mike Coble, Robin Cotton, and Charlotte Word Mid-America 2013 Forensic DNA Conference Sponsored by Paternity Testing Corporation Instructors: various speakers GeneMapper ID-X Training Sponsored by St. Louis County Police Crime Laboratory Instructor: April Orbison 3500 Genetic Analyzer Install Training Sponsored by St. Louis County Police Crime Laboratory Instructor: April Orbison Mid-America 2012 Forensic DNA Conference Sponsored by Paternity Testing Corporation Instructors: various speakers Workshops: Introduction to CODIS 7.0, Instructor: Melody Josserand Paternity Testing for Crime Laboratories, Instructor: Michelle Beckwith DNA Mixture Interpretation Workshop Sponsored by St. Louis County Police Crime Laboratory Instructor: Bruce Heidebreeht 02:12 1554fh Annual Scienti?c Meeting of the American Association of Forensic Updated 10/7114 Page 3 of 7 08f11 06:11 11110 092?10 07f10 07/10 06/10 04110 10:09 Updated mama Sciences (AAF S) Sponsored by AAF Instructor: various speakers Preparation Course Sponsored by Instructor: Anja Einseln 2011 NIJ Conference Sponsored by theNational Institute of Justice Instructors: various speakers 16?] Annual National CODIS Conference Sponsored by the Federal Bureau of Investigation Instructors: various speakers Forensic Training Sponsored by Marshall University Instructor: Sarah Bowen Introduction to SQL Intermediate Applications of SQL Introduction to Reports - Sponsored by the University of MissourinSt. Louis, Computer Education and Training Center Instructor: Sandy Lux Quality Assurance Training Sponsored by the U.S. Department of Justice, Federal Bureau of Investigation Laboratory Division Instructor: Onl-inc self?paced training 2010 NIJ Conference Sponsored by the National Institute of Justice Instructors: various speakers Mid-America 2010 Forensic DNA Conference Sponsored by Paternity Testing Corporation Instructors: various speakers 2009 NIJ Grant Management Summit Sponsored by the Investigative and Forensic Sciences Division, the Of?ce of Science and Technology, the National Institute of Justice and the US. Department of Justice Instructors: various speakers Page 4 of? 06109 04109 03:09 04103 03:?08 10107 06107 03:07 03ft]? Updated 1 0/7114 Advanced DNA Training Sponsored by NIJ President's DNA Initiative and Marshall University Instructor: Justin Godby To Hell and Back: The Ethics of Stewardship and the Stewardship of Ethics Sponsored by the National Institute of Justice - Instructor: Paul J. Voss, Bloodstain Pattern Analysis for DNA Scientists Sponsored by the St. Louis Metropolitan Police Department Crime Laboratory Instructors: Kevin R. Winter and Michael J. Van Stratton Mid-America 2008 Forensic DNA Conference Sponsored by Paternity Testing Corporation Instructors: various speakers Screening Hair for DNA Sponsoredby St. Louis County Police Department Instructor: William Randle 18th International Symposium on Human Identi?cation Sponsored by Promega Instructors various speakers Validation workshop John Butler, Expert Witness Testimony workshop Charlotte Word and George Clarke Hair Identi?cation for DNA Sponsored by West Virginia University and Midwest Forensics Resource Center Instructor: Dick Bisbing Mid~America 2007 Forensic DNA Conference Sponsored by Paternity Testing Corporation Instructors: various speakers Continuing Education for Forensic Professionals Sponsored by National Institute of Justice and West Virginia University Forensic Science Initiative Instructors: various speakers Expert Testimony Workshop Ethics in Forensic Science Scienti?c and Technical Writing FBI CODIS version 5.7.3 Software Training Sponsored by Federal Bureau of Investigation Instructors: Meghan Carlin and Inez Kendall Page 5 of 7 11;?06 Forensic Statistics: The Calculations Behind PopStats and Beyond Sponsored by Missouri Association of Crime Laboratory Directors Instructor: John Planz, 10206 04:06 10f05 03f05 02I05 Courtroom Testimony Techniques: Success Instead of Survival Sponsored by Midwest Forensics Resource Center Instructor: Dwane Hilderbrand Mid-America 2006 Forensic DNA Conference Sponsored by Paternity Testing Corporation Instructors: various speakers Paternity Statistics Workshop Instructor: David W. Gjertson 7 Forensic DNA Statistics Workshop Instructor: George Carmedy Midwestern Association of Forensic Scientists Annual Meeting Sponsored by: Midwestern Association of Forensic Scientists Instructors: various speakers Genelnapper ID Software Workshop Instructor: Erica Currie-Fraser Real-Time PCR Workshop Instructor: Catherine Caballero Mid-America 2005 Forensic DNA Conference Sponsored by: Paternity Testing Corporation Instructor: various speakers Forensic Statistics Workshop Sponsored by: Paternity Testing Corporation Instructor: Charles Brenner Forensic Statistics Workshop Sponsored by: St. Louis Metropolitan Police Department?Crime LabOratory Instructor: Donna Becherer Papers Presented 2014 2014 2014 2013 Updated 1 oar/14 Giobai?ier Casework and Express Kits Validation. Presented at the Summer 2014 Association of Forensic DNA and Administrators Meeting in Globalfiier Casework and Express Kits Validation. Presented at the 2014 (July) Future Trends in Forensic DNA Technology Seminar in GIobal?Ier Casework and Express Kits Validation. Presented at the 2014 (April) Mid?America Forensic DNA Conference in Casework Experience with Erase-Update. Presented at the 2013 Mid-America Forensic DNA Conference in Page 6 of? 2012 Casework Experience with Erase. Presented at the 2012 Mid?America Forensic DNA Conference in - 2008 Minifiler: Designer Primers for the Degradation Blues. Presented at the 2008 Mid~Ameriea Forensic DNA Conference in 2003 Reconstruction of Prehistoric Hurrian Dietary Patterns by Laser Abiation ICP-MS. Presented at the annuai Society for American Archaeologists meeting in Milwaukee, WI. 2002 Laser Ablation of Missouri Cheri. Presented at the MURR Nuclear Sciences Research Symposium in Columbia, MO. References upon request Updated 1 0/7/14 Page 7 of 7