http://mrmc.amedd.army.mil/content/foia_reading_room/Batch77/20070521_batch77(red acted).pdf https://mrmc.amedd.army.mil/content/foia_reading_room/Batch78/20070611_batch78(re dacted).pdf 'I'tlt'. FE: Wilt.? Emma? Dill! Vim. Jun! IS. 2113? LELE-JH PH Classr?ceunn'. UNCLASSIFIED CaveeE: MDNE Fwe rnega'eds she-dd have been rmre tha'l emugh 11:: hi everything. but the sanples Iwere nimreds. Iddn?twa?tme smres tube Fried erther.endtne tuteldf Eu megemtls rut a ernateng. In the past, A rr'egereds INDUH due 1he job. This Is mien reds ever that. end n: sterility. Maybe we need ta an stenllt'g.I :LIrue 'n'ltl'l 11: see Hat the dIe-dtf rs Inn'eeslng mis?t?) - Tn: I Bruce Dr USN-NEIL Ct: Jeni: :Irmdletrm I UNCLASSIFIED meets: MDNE Irl'tat :5 gang the marketer? let rut pruned-r?" Th-Ee things HE going td he fned to Lnsp! Mange-m- l-rdrn: Iu1ns. Emce Dr USAMRIID Sent: Wednade June 13 Salim: 3 (UNCLASSIFIED) Classi?caudn'. UNCLASSIFIED Caveats: NCINE The sermles {see file} 'n'EfE rmdleted an SD HAT W. math 5 Megs-Eds. The In'ere ?hnt." an JJH DI. they were re-Inadleted hl'?'l en 1 reds [1 Hegered}. Eighteen 0f the 2? EMF-IE we tested are "hdt." They En?t he released until thefre sterle. SD :5 gang to glee the 1het were net the ones that weren?t medeced} HI be re-In'e I with en 2 Hegereds. Hdpel?ulty this Stay Hal-n: I?m-r; ME Gr LEAHHZIEI PW: rub-re ban: Ind-Him nan-I Dill: Hustle-r. hull. am mm Claim-Latin". Caveats: NONI: retumed 1he remaining 25 EETIFEE after 2 rnegemtls GT Inathatlnn. it: gram. Mange-m- I-rnrn: Inns. time I: Dr Sent: Thursda line 1-1 mm AH Tn: ]u1I15. HnJce T: Elr JEUII More bed Immatm news Classt?ca'hen'. Caveats: Milt-II: Atterreche? nf?'le 5 singles that aspearetl 1n shed-ecarteramt?ef? reds. arty two negate-e- The ~55, J'Eanrl SIB-came mmt. Orig Menu Izlun?t need to be Irrathatetl agan- Hssageum I-mrn: 1u1ns1 Bruce I: Dr Sent; Wednade June 13 31!] PM TD: Jed: Jn'a Iatnn stenlzatnn Classt?eaten'. Caveats: MDNI: Please rate the new meadsheet- were tested en San. The numbers In red were still for E. anthems The nurbers blue Iwere negatwe for E. anthems~ The negats'e sa'nFles lwere re- platetl 11: make sure hey are negatwe. Dara LEAHHZIEI 'I'ul. Wt hm be: I'm rim-I Tram.m1?, AH Uaseu?eaunn'. UHCLASSLHED Caveats: ?tter Elf 1he E- sarr'des that mpeared 1n pes5 stain,- [heck after a mtaef E: reds. arty two auntiesremuled regent-e- Tl'e ntherfnu -5E. 63, J?Band BIB-came wit-101i}! 9-H mm reed ta be marketed agan- Emce -?--Ongml Masageum Mus. Sent: Wednade Jute [3 313 PM Tn: Ject: W: tn'a Iatnn etenlzatnn Ua?l?eatnn'. UNCLAEELHED Caveats: NONE Please note the new spreadsheet. dam-QC were tested on SEA. The numbers In red were still for El. antt-?ans gram. The mum-ere blue were negetwe for El. antt-?ane The regauue aunties were re- platetl 1n 5ure 111e'3.I a'e negative. Emce -?--Cngral Mange-m- 1-.nn5. Lew-1mm Sent; Wednade June 13 1t135Ar-1 Tn: abject: li??lEIlI?'l 5tth ure..-agem Elasst?lzamn'. UHCLASSLHED Caveats: Milt-II: Tl'e 23" sarrdes [see ?le} were mediated en MM math 5 Mega-115. Tl'e EHHFIES were Film: I?m-r; B'uu; Gr LEAHRZIEI 'I'nr. W: Pam ta: rut-s Dill: Hustler. mm, mm ?asl?taunn; Caveats: NONI: 115: retumed 1i1e remaining 25 sanples after 2 megamcls sf Irranhatlnn. IIEILHZEIJ HI test far gray-m. Mange-m- I-rnrn: nuns. Bruce I: Dr Sent: Thursda 1-1 mm AH Tn: Mn; Bruce I: Dr Jed: More bed Immatm Hens Elam?eaunn'. Caveats: NONI: Hfterred?ed: nf?'le sarrdes that aspearecl 1n Lreduafteramtaaf? reds. arty Mn sanplesrerniled negause- The ntherfnu' -5E. 63, J'Band EMS-came Lunar. Orig Mind Um?: need be be marketed again. Hssegeum I-rnrn: 1u1ns. Emce I: Dr Sent; Wednasa June 13 3:38 PM Jed: I-W: irra Iaten stenlzahm ?asl?taunn; Caveats: NONI: Please nate the new spreedsl'leet. 45-91: were tested en San. The numbers In red were still fm El. anti-tans gray-m. The name-rs blue lwere negative for El. anti-rams. The negauve samples Iwere re- ?atecl 11: make sure mew e'e neuetwe. Ct.- ?Hit! mumnm mma:mm UHCLAEEIHED Eaveats: MDHE and H3 mare Imdlated Huh IHr-un Ra?sm 11' JJN El}. There are mrremhl 9 rli heameEterda'r i'ld Sh 3'93. the ?ates were Harri-led i'ld the-,- are negaErue. The-9?" in?atedwer meneeltem. We daft have emulate an the ether EMF-IE {the furl. Hupel?ulhr thefll heck negatnle. EFLHIE as: on: UN LASEIHED Caveats: HUME 'I'ne ?he'll! E?'lln?tbn MMFEEW Duh! ?Burl-.141! B. 2113? mm: Elm?cauun: UNCLASSIFIED Caveats: MDHE The send [see ?le) we radiated an 31} MAT M. math 5 Mega-115 The samples Iwere ?hc-L? an 1 JUN Eli. the-,- were re-Inadlated H111 an additional 1 red: [1 Eighteen {If he 2? Emma were teeted and 12 {If 111E are "hut." The-g.I aft be released thefre sterie. I5 gang ta gwe the Emma met were mt the DIE 1het weren't deduced]- wl be re-na I with en 2 Mega'eds. Hopefully this wen-k. Stay brute a Eur-en: LHSSIFIEEI Caveats: From- m: HE: mum mama ?LII-mam WM. Ln! L1. Elast?eatnn: meets: HUME ?nd nhat?snnt dl that left. 5r, - I't Tn: 1u1n5. EI-rute [Jr new-1mm RE: [naiathn 5tth ?alure..-agem Um?eaum; UHCLAEELHED Caveats: MDHE SEITE ?ung I5 net nght. Maegan?- From: Bruce Elr USAHMID Sent: Wednada June 116 PH Um?eaum; UHCLAEELHED Caveats: MDHE Five rnega?ads she-dd have been me tha'l Hugh kl euery?'llng. But the samples were suhntafterm edit-anal 1 urban reds. Idan?twat?'le mes tube Fried ear-enamel the Intel at rhegamds mt 5 amazing. 1n the past, 4 negarads maukl dc: '?1e pb. I5 2 when reds ever that. and 5t! Maybe we need ta a ch spam 11: 5e mat the dle-nf?f IS with Inn-e35an r-1 Pram: Sent: Wednatlagr. June 1MB AM To: tum Bruce Dr Er. Jed: 5tth Queers: HDNE 'Mat I5 the Irratiatnr? marking paw-e115? Thane 11:: he fned ta 3 map! Mange-m- Fmrn: lu1l15. Emce SdJJect: 5tth Classl?taunn'. UNCLAEELHED Caveats: HCIHE The 1? games {see flle} Inlere [mutilated an Hm W. math 5 Mega-115 The samples I?were 5t- ?hnt." 5D an ll JUN El}. the-Ir were re-Inatllated an addmunal 1 reds [1 Eighteen cf the 2? imp-IE we tested In and 12 Elf ?'le [El are 5t- "hnt." Isn't be released untll tnefre meme. I5 gang ta glee the 1113': we mt [pins the cues that Inlerven?t medeced} 'nl be re-Ira I with en 2 Mega'ade. Hupefulty Wk. Stay tuned.-. - :uru:e Brute nuns a LASSIFLED Caveats: MIDI-IE Cl?l?eauun; UNCLAESLHED Caveats: MIDI-IE reading r00m/Batch79/2007O716 batch79(red From: To: [In] [El] Subject: HM: Spore Paperwork Dabs: Thursday, August DEL 200? 3:16:21 AM Classi?cation: UNCLASSIFIED Caveats: NONE HI gave all the information to who is out on medical leave after being injured in a fall. on't have copies of the work re In Ice. I know that several rounds of inadiation had to be clone to ?nally; get all of the spores sterile. It seems as though the new inadiator isn't as reliable as the old one for some reason. -bruce Bruce Ivins inal Mes From:m Sent: av, ugust :02 PM To: Ivins, Bruce Dr USAMRIID Subject: Spore Paperwork Classi?cation: UNCLASSIFIED Caveats: NONE Bruce, Would it be possible to get the sterility paperwork for the 2? spores we are tsting for Thanks Caveats: NONE Classi?cation: UNCLASSIFIED Caveats: NONE reading r00m/Batch85/20080312 batch85(red From: Tu: Subject: Irradiated spore; - transFEr Data: Wedn?day, March 12,. EDGE 12:23:01 PM Classi?cation: UNCLASSIFIED Caveals: NONE and I Shave talked about it at a division meeting a few weeks a I want to tell you that we will be transferring 5 ml of Arnes res Select Agent number 12538, to Ten ml of the mores were irradiated by wi?'l 4 Megarads. As discusse at mee?ng. 50% [5 ml of the material was checke or sten It); on BA, and there was no 43me at all. You may have seen?J insistence that we need only check 10% of the material for sterility, but I think that a Si: ec or B. anthracis samples is a good idea. We've had in the past some samples that failed sterility checks, so 50% leaves us "better safe than sorry." -lJruce Bruce Ivins reading r00m/Batch75/20070324 batch75(red acted From: To: Subject: Filli': Given to for Inediation Date: Fnizlaiy. March 30, 2130? 1:35:42 PM Classi?cation: UNCLASSIFIED Caveats: FOLIO Here is the record for the sterility,r check. Ten percent of the total material was plated out onto SBA and it was all negative for growth. The plates, incubated over the weekend, remained negative. Bruce Ivins From: lvins, Bruce Dr Sent: Frida November 133 2005 9:44 AM The spores were irradiated on Dctoher so. on November am plated out 10% of each of The preps onto SBA plates. All were negative for growth 24 hours ater. ev be incubated over the weekend. Bruce Ivins The following spore preps were given tom for irradiation [4 Mega Raids}: 1. Arnes, Select Agent H49, made at USAMRJID in Leighton and Do: medium in shaker ?asks, then pun?ed 2. Arnes, Select Agent at 11335 made at USAHRIID on SBA plates, not puntied 3. Arnes, Select Agent 439, made atm in Leighton and Bar medium in fen?nentors, not pun?ed 4. Arnes, Select Agent moo, made at Il'l Leighton and Del medium Il'l fermentors, then puri?ed 5. Delta Sterne Sperm - made in shaker flasks in E- medium, then puri?ed - "clean" material {separated from dirty dehns?l reading r00m/Batch74/20070211 batch74(red ject: Importance: High Heyr Gang- transfer of agents (LE. to for irradiation, or aerobiology, etc.}. Below is an excerpt from our BACT SOP. I have also attac two samples of forms that should accompany such transfers. Please have this paperwork approved prior to performing an inter-divisional transfer of agents. Cine item that has come uE during our inspection is consistency of our records for inter-divisional Also, we need to have better record keeping of sterility checking procedures. Please document the sterility check procedures you used ?v'olume plated, incubab'on time, and result of check (PASS 0R FAILJJ. If we can keep the transfer documentation and the sterility check documentation toge?'ler in a notebook, that would be ideal. Thanks for your help with this! Inter-Divisional transfer. Authorization. A written request must be received and endorsed by them prior to the transfer of infectious material. A copy of this request will be provl to responsl P.I., the original will be maintained in the Division office. The transfer of infectious material must be conducted properly IAW with this SOP and documented. The sender?s cold side reprSentative must 1.risually con?rm that a known recipient picks up the material. The hot-side sender will then document the transfer via the air-lock log then follow-up wi?'l an e-mail to the receiver. Once the receiver acknowledgE receipt the e-rnail should be achieved. The log and e-mail should contain the following elements of information: Date, form and duanb'ty of infectious material, IGenus, species, strain, sender name, recipient's name, destination and purpose of transfer. reading r00m/Batch69/20060626 batch69(red To: Subject: Irradiated Data: Wedngday, July 19, 2006 9:59:13 AM Fourteen ml of Select Agent l?49 Spores, were inadiated wi?'l 4 MegaRads on June 30, 2006. Ten Sheep blood agar plats were inoculated with ELI ml per plate of the irradiated spores. The plates were incubated at No grow?'l was SEED on any of the plata after 24 or 3?2 hours incubation. Bruce Ivins