CC: A. .J. DuhKarrh - 3:11:00
  L. K. l?apa - 209
l? Pral File

E. I. DU Pom oc NEMOURS COMPANY 

inCM'G?l'to

WILMINGTON. DELAWARE 19898

POLYNCER PRODUCTS. FTMF N1

Exp??l??cNt 5 I .r-N

PERSONAL AND CONFIDENTIAL
July 16, 1981

T0: DR. A. BINNERTS - PPD, Dordrecht

FROM: 5. s. STAFFORD QS?szun/?J

ANALYSIS OF BLOOD SAMPLES FOR PERFLUOROOCTAEOATE
(Job No. PRAL Nos. 
81-2613-2618; Notebook Nos. 

As requested in your letters of 5/20, 5/26, 6/2, and 6/10/81 to L. J. Papa.
the 21 blood samples Submitted then have been analyzed for perfluorooctanoate
(C8). Results and sample identification are given in the attached table.

As noted there, the analyses were done using a gas chromatographic
method specific for C8 (Lab Method Number 55-567) but results have been reported
as for comparison with total organic fluorine analyses. Precision is 102
relative standard deviation over most of the concentration range, somewhat less
at the lowest values- The lower limit for quantitation is 0.007 (0.01 
perfluorooctanoic acid), with a detection limit of 0.004 which can be dis?
tinguished from the reagent background but not well quantitated.

Please contact me (772-4h?0) or L. J. Papa (772?2745) if you have any
questions regarding the analyses. General questions on blood sampling can be
directed to J. K. Raines or L. F. Percival.

Attachment
jah

Key Words:
Periluorooctanoic Acid
Perfluorooctanoate
Blood Analysis
GC

mur? 5-. weak} or Ilungs 09mg sun-t Unfit; at-om


310713847

 

 

TABLE I

CONCENTRATION OF PERFLUOROOCTAKOATE IN BLOOD 

 

 

 

 

 

 

Sample CC Analvsis (L
PRAL No. Date Sampled ?g?g Date Analvzed ug_F/g blot
81?2239 5/19/81 04392 6/4/81 1.4
81-2240 5/19/81 03240 6/4/81 1.4
81-2241 5/19/81 03789 6/8/81 8.1
81-2262 5/19/81 04822 6/3/51 3,6
81-2243 5/19/81 03370 6/8/81 4.5
81-2393 5/26/81 03824 6/10/81 7.8
81-2394 5/26/81 04841 6/10/81 5.9
81?2395 5/26/81 01999 6110/81 6 6.6

6116/81
81-2396 5/26/81 03726 6/10/81 6.6
81-2397 5/26/81 02978 6/15/81 2.6
81-2530 6/2/81 04793 6/9/81 6

6/16/81 20.
81?2531 6/2/8l' 04108 6/9/81 6.5
81?2532 6/2/81 '04499 6/9/81 3-9
81-2533 6/2/81 03859 619/81 9.2
81?2534 6/2/81 01611 6/9/31 4.9
81-2613 6/9/81 01530 6116/81 4.8
81-2614 6/9/81 01951 6116/81 0.59
81?2615 6/9/81 03293 6/16/81 3.6
81?2616 6/9/81 02180 6/16/81 4.1
81?2617 6/9/81 04502 6/16/81 7.7
81?2618 6/9/81 02141 6/16/81 2.1

(3) Analysis as described in Lab Method ES-567
Acid in Blood, Gas Chromatographic Method",
packed column GC analysis with perfluoro?n?octanoic ac

 

("Determination of Perfluorooctanoic
S. Stafford, 4/3/81). using the
id as calibration standard.

Although the analysis is specifically for perfluorooctanoate (acid or salts),
concentrations are given in fluorine for comparison with the results of

total organic fluorine analyses.
Estimated uncertainty is 10% relative standard deviation.
for quantitation is 0.007 ugF?g.

concentrations in that range canno

0.007. None detected is reported for samples with

which cannOt be distinguished from reagent backgrOund.



(ppm 0.688 perfluorooctanoic acid)
The lower limit
The detection limit is 0.004 ugF/g, but
be well quantitated and are reported as
0.004 ppm,

'u



EID713848

 

4-.-.