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ru IlIECJ-STD-O 604.00
DECEMBER 1978

PREllMIN

STANDARD

U.s. DEPARTMENT OF JUSTICE
Law Enforcement Assistance Administration
National Institute of Law Enforcemtjnt and Criminal Justice

  

x. in ii?tl  
..7 . ?4 c1 .fx .111LAW ENFORCEMENT STANDARDS PROGRAM

NILECJ STANDARD
FOR
CHEMICAL SPOT TEST KITS FOR
PRELIMINARY IDENTIFICJATION OF
DRUGS OF ABUSE

A Voluntary National Standard Promulgated by the
National Institute of Law Eniorcemeiit and Ciiminal Justice

DECEMBER 1978

u.s. DEPARTMENT OF JUSTICE
La\.., Enforcement Assistance Administration
National Institute of Law Enforcement and Criminal Justice

 NATIONAL INSTITUTE OF LAW ENFORCEMENT
AND CRIMINAL JUSTICE
Blair G. Ewing, Acting Director

LAW ENFORCEMENT ASSISTANCE
ADMINISTRATION
James M. H. Gregg, Acting Administrator

ACKNOWLEDGMENTS
This standard was formulated by the Law Enforcement Standards Laboratory of the National Bureau of
Standards under the direction of Robert M. Mills, Manager, Investigative Aids Program, and Jacob J.
Diamond, Chief of LESL. Technical research was performed by the staff of
NBS Analytical Chemistry
Division under the supervision of Dr. Rance A. Velapoldi. The standard has been reviewed and approved by
the National Advisory Committee for Law Enforcement Equipment and Technology of the International
Association of Chiefs of Police and adopted by them as an IACP standard.

For sale by the Superintendent of Documents, U.S. Government Printing Office
Washington, D.C. 20402
Stock Number 027-000-00730-9

 NILECJ STANDARD
FOR
CHEMICAL SPOT TEST KITS FOR PRELIMINARY
IDENTIFICATION OF DRUGS OF ABUSE
CONTENTS
Page

Foreword ................................................. , . . . . . . . . . . . . . . . .
1. Purpose ................................................................
2. Scope..................................................................
3. DefInitions..............................................................
4. Requirements ..............................'..............................
4.1 User Information ... . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4.2 Labeling ............................................................
4.3 WorkInanship ................. '.. .................. ...... ............ .
4.4 Safe-Disposal Materials ............•............................. "....
4.5 Color Samples ............................ ,..........................
4.6 Final. Color' '....................................................... , . .
4.7 Sensitivity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
4.8 Detection Limit ... ".................................. ,................
4.9 SpecifIcity .................................... , ... , ' ............ , . . . .
5. Test Methods ...................................... , . . . . . . . . . . . . . . . . . . . . .
5.1 General Test Conditions. ... . . . .... ..... ..... .. .. ..... . ........ ..... ...
5.2 Color Sample Test . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.3 Final Color Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.4 Sensitivity Test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
5.5 Detection Limit Determination ................ ,... . ........ ..... .. ... . .. .
5.6 SpecifIcity Test ................. , . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .
Appendix A ............. , , . . . . . . . . . . .. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ... . . . . . .

iii

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7

 FOREWORD
Following a Congressional mandate I to develop new and improved techniques,
systems, and equipment to strengthen law enforcement and criminal justice, the National
Institute of Law Enforcement and Criminal Justice (NILECJ) has established the Law
Enforcement Standards Laboratory (LESL) at the National Bureau of Standards. LESL's
function is to conduct research that will assist law enforcement and criminal justice agencies
in the selection and procurement of quality equipment.
In response to priorities established by NILECJ, LESL is (1) subjecting existing
equipment to laboratory testing and evaluation and (2) conducting research leading to the
development of several series of documents, including national voluntary equipment
standards, user guidelines, state-of-the-art surveys and other reports.
This document, NILECJ-STD-0604.00, Chemical Spot Test Kits for Preliminary
Identification of Drugs of Abuse, is a law enforcement equipment standard developed by
LESL and approved and issued by NILECJ. Additional standards as well as other
documents will be issued under the LESL program in the areas of protective equipment,
communications equipment, security systems, weapons, emergency equipment, investigative
aids, vehicles and clothing.
This equipment standard is a technical document consisting of performance and other
requirements together with a description of test methods. Equipment which can meet these
requirements is of superior quality and is suited to the needs of law enforcement agencies.
Purchasers can use the test methods described in this standard to determine firsthand
whether a particular equipment item meets the requirements of the standard, or they may
have the tests conducted on their behalf by a qualified testing laboratory. Law enforcement
personnel may also reference this standard in purchase documents and require that any
equipment offered for purchase meet its requirements and that this compliance be either
guarar.teed by the vendor or attested to by an independent testing laboratory.
The necessarily technical nature of this NILECJ standard, and its special focus as a
procurement aid, make it of limited use to those \vho seek general guidance concerning
chemical spot test kits. The User Guide Series is designed to fill that need. We plan to issue
guides to various items of law enforcement equipment as soon as possible, within the
constraints of available funding and the overall NILECJ program.
The user guides being issued are highly readable and tutorial in nature in contrast to
the standards, which are highly technical and intended for laboratory use by technical
personnel. The guides provide, in non-technical language, information for purchasing agents
and other interested persons concerning the capabilities of equipment currently available.
They may then select equipment appropriate to the performance required by their agency.
Recommendations for the develupment of particular guides should be sent to us.
NILECJ standards are subjected to continuing review. Technical comments and
recommended revisions are invited from all interested parties. Suggestions should be
addressed to the Program Manager for Standards, National Institute of Law Enforcement

I

Section 402(b) of the Omnibus Crime Control and Safe Streets Act of 1968, as amended.

v

 and Criminal Justice, Law Enforcement Assistance Administration, U.S. Department of
Justice, Washington, D.C. 20531.
LESTER SHUBIN

Program Manager for Standards
National Institute of Law
Enforcement and Criminal Justice

vi

 NILECJ..STD-0604.00

NUJECJ STANDARD
fOR

CHEMICAL SPOT TlEST KIYS fC)R PRELIMINARY
OF lIlRUC;p§ Of ABUSE
1.

PURPOSE

The purpose of this standard is to establish minimum requirements for chemical spot
test kits and methods of testing the kits to determine compliance with those requirements.

2.

SCOPE

This standard applies to field testing kits which use chemical spot tests for the
preliminary identification of drugs of abuse (hereinafter referred to simply as drugs). It does
not apply to kits which use thin layer chromatography as the identification procedure nor to
kits which identify drugs in body fluids.
Note that this standard does not mandate the identities of the reagents to be included
in a test kit. The eteven reagents listed in appendix A and their color reactions as listed in
table 1 are included only as a convenience, since they are among the reagents currently in
most common use. A kit may contain any reagent or group of reagents that meet the
requirements of this standard.

3.

DEFINITIONS

3.1 Centroid Color Charts
A collection of charts, published by the
Color Council and the National
Bureau of Standards, which logically group and illustrate the colors. There is a chart for
each color hue. On each chart, color saturation increases from left to right and color
lightness increases from bottom to top. The charts are identified as NBS Standard Reference
Material 2106 and are described in NBS Circular 553. The charts may be purchased from
the Office of Standard Reference Materials, and the circular may be obtained from the
Office of Technical Publications, both at the National Bureau of Standards, Washington,
D.C. 20234.

3.2 Color Vicinity
Those colors, other than the greys, which are the same as or are adjacent to the
referenced color in a Centroid Color Chart. For example, colors in the color vicinity of
number 15 on the Red-Pink chart are numbers 3, 6, 12, 13, 15, 16, 18, 19 and 20.

3.3 Final Color
The color remaining after any intermediate colors, produced by the addition of a
reagent to a drug or other substance, have disappeared.

 4.

RIEQUIREMENTS

4.1 User Information
The kit shall include the following information.
4.1.1 Drugs Detected

A list of the drugs for which the reagents in the kit can be used to make a tentative
identification with acceptable specificity. This list shall consist of those drugs listed on the
reagent containers in accordance with 4.2.b.
4.1.2 Instructions

Clear instructions for performing the chemical spot tests and for interpreting the results.
4.1.3 Safety Precautions

a) Warning of the hazards of the flammable aud corrosive chemicals contained in the
kit.

b) Steps to follow and antidotes to u:::.e if hamrdous reagents are taken internally or
come in contact with parts of the body or clothes.
c) Procedures for safely discarding used reagents and containers.
4.1.4 General

a) A statement that the kit is intended to be used for presumptive purposes only, and
should not be used for evidentbl purposes unless the results are verified by a qualified
forensic scientist in a properly equipped crime laboratory.
b) A statement that users of the kit should receive appropriate training in its use, and
should be taught that the kit can give false positive as well as false negative results.
c) A discussion of cleaning and the use of clean apparatus to decrease the probability
of reagent contamination and misleading results.
d) A discussion of proper kit storage in buildings and vehicles.

4.2 Labeling
Each reagent container shall have a label which either directly or by reference:
a) Identifies the reagent.
b) Identifies the drug or dnJo<; which it can detect.
c) Is prominently marked "Danger," where appropriate.
d) Gives a discard date, where appropriate. Serial numbers, if printed on the labels,
satisfy this requirement (4.2.d) if discard dates corresponding to those serial numbers are
given elsewhere in the kit.

4.3 Workmanship
Visual inspection of the kit shall show no broken or inoperative catches, hinges or
containers. There shall be no evidence of reagent leakage.

4.4 Safe-Disposal Materials
The kit shall contain chemicals for neutralizing strongly acidic and basic reagents and/
or acid/base resistant containers into which used reagents and containers can be deposited
:-nd safely disposed of at a later time in accordance with 4.1.3.c.
2

 4.5 Color Samples
4.5.1

The kit shall include samples or reproductions of the final colors
by each
reagent in the kit when reacted with each drug listed on the reagent container label.
4.5.2 If Reagent is Listed in Appendix A

For each reagent in the kit which is listed in appendix A, the color samples shall be in
the color vicinity of the appropriate final color listed in table 1, when matched in accordance
with 5.2.
4.5.3 If Reagent is Not

in Appelndix A

For each reagent in the kit which is not listed in appendix A, the color samples shall be
in the color vicinity of the color
by the manufacturer in accordance with 4.6.2,
when matched in accordance with 5.2.

4.6 Final Color
4.6.1 If Reagent is Listed in Appendix A

Each reagent in the kit which is listed in appendix A shall produce a final color which
is in the color vicinity of the appropriate final color listed in table 1, when tested in
accordance with 5.3.
4.6.2 If Drug/Reagent Combination is Not Listed in Table 1

The manufacturer shall specify the Centroid Color Chart color which most nearly
matches the fmal color of each drug/reagent combination listed on a reagent container label,
but not included in table 1.
4.6.3 If Reagent is Not listed in Appendix A

Each reagent in the kit. which is not listed in appendix A shall produce final colors
which are in the color vicinity of the fmal c!)lors specified in accordance with 4.6.2, when
tested in accordance with 5.3.

4.7 Sensitivity
4.7.1

Each reagent in the kit shall produce a fmal color which is either in the color vicinity
of, or has the same color hue and color saturation P.s the final color listed in table 1 or
specified in accordance with 4.6.2, for each of five replicate tests, performed in accordance
with 5,4 at the drug detection limit listed in table 2 or specified by the manufacturer in
accordance with 4.8.

4.8 Detection Limit
The manufacturer shall specify the drug detection limit, determined in accordance with
5.5, for each drug/reagent combination listed on a reagent container label, other than those
listed in table 2. For any drug listed in table 3, the limit shall not exceed that listed in table

3.

4.9 Specificity
The kit shall include sufficient reagents to permit differentiation between each drug
3

 listed in accordance with 4.1.1 and the other substances listed in table 4. The differentiation
may be accomplished by the use of a single reagent, or by a combination of reagents.
Acceptable differentiation occurs if the final colors of the tests are not in the color vicinity
of one another when checked in accordance with 5.6.

5.

TEST METHODS

5.1 General Test Conditions
At the time of the tests, the ambient temperature shall be between lOoC and 40°C (SOOF
and HWF); the relative humidity shall be between 10 and 90 percent.

5.2 Color Sample Test
Visually compare each color sample in the kit with the colors in the Centroid Color
Charts, and determine whether the color in the Centroid Color Charts which most nearly
matches is in the color vicinity of the appropriate fmal color.

5.3 Final Color Test
5.3.1 Drug Selection

Select drugs as follows for the deterrdnation of the final colors developed by the
reagents:
5.3.1.1

For each reagent in the kit which is listed in appendix A (except A.S), select any drug
listed on the reagent container label which is also listed in table 1 and asterisked.
5.3.1.2

If reagent A.5 is in the kit, select one drug from each of the three following groups:

Group I: Codeine Sulfate, Heroin.HCI, Morphine.
Group II: D-Amphetamine.HCI, D-Methamphetamine.HCI, Mescaline Sulfate.
Group III: Any other drug listed on the reagent container label.
5.3.1.3

Test each reagent in the kit other than
on the reagent container label.

listed in appendix A with each drug listed

5,3.2 Reference Final Color

Find in the Centroid Color Charts the appropriate fma1 color listed in table 1, or the
color specified, in accordance with 4.6.2, by the manufacturer.
5.3.3 Test Procedure

Place in the test container an amount of the pure drug which is between one and ten
times the drug detection limit but no less than 25 fLg.
5.3.4

Add reagents in the specified volume and order, or break sealed glass tubes in
4

 accordance with the kit instructions. Note the final color, which in almost all cases will be
produced within two minutes after the last reagent is added.

5.3.5
Compare the final colors produced to those in the Centroid Color Charts, and
determine the color in the Centroid Color Charts which most nearly matches.

5.3.6
Determine whether the Centroid Chart color referenced in 5.3.5 is in the color vicinity
of the color referenced in 5.3.2.

5.3.7
If either 4.6.1 or 4.6.3 is not satisfied, check the drug solution and test procedure by
repeating 5.3.3 through 5.3.6 using fresh reagent prepared as directed in appendix A or by
the manufacturer. This paragf'"dph (5.3.7) is not applicable for reagents not listed in appendix
A unless information similar to that in appendix A is supplied by the manufacturer.

5.4 Sensitivity Test
5.4.1
Use the same drugs for sensitivity testing of each reagent in the kit as for the final
color test (5.3).

5.4.2
For marijuana, transfer a quantity equal to the drug detection limit into each of five test
containers and proceed to 5.4.6.

5.4.3
'prepare 1.0 p,glfJ,,} solutions of the other selected drugs in reagent grade solvents.
Chloroform and distilled water are convenient solvents for the drugs listed in table 2, as
indicated.

5.4.4
For each drug, transfer by micropipette into each of five test containers the volume of
drug solution, in p,l, numerically equal to the drug detection limit, in p,g, listed in table 2 or
specified by the manufacturer in accordance with 4.7.2.

5.4.5
Evaporate the solvent by infrared heating and/or by gently blowing a stream of gas
such as oil-free air, nitrogen, helium, etc., over the surface of the solution. Keep heating
time to a minimum by heating only until the solvent is evaporated, to avoid the possibility
of drug decomposition. The temperature of the solution should not exceed 40°C (104°F)
during solvent evaporation.

5.4.6
Add reagents in the specified volumes and order, or break sealed glass tubes in
accordance with the kit instructions.

5

 5.4.7

If one or more of the five replicate tests results in a final color which is not in
accordance with 4.7.1, check the drug solution and test procedure by repeating
(5.4.2
for marijuana) through 5.4.6 using fresh reagents prepared as directed in appendix A or by
the manufacturer. This paragraph is not applicable for reagents not listed in appendix A
unless information similar to that in appendix A is supplied by the manufacturer.

5.5 Detection Limit Determination
Prepare a solution, using a suitable reagent grade solvent, having a drug concentration
of 1.0 JLg/,.,J, or lower if necessary. Using a micropipette, transfer 5 samples of a convenient
volume of this solution to the test plates. Evaporate the solvent and add reagent, as in 5.4.5
and 5.4.6. Change the quantity of drug transferred to the test plate by varying either the
solution concentration or the volume transferred, and repeat the test until the smallest mass
of transferred drug is determined, to one significant figure, for which five out of five color
changes are observed. As a safety factor, mUltiply this '!uantity by ten, and use the product
as the operational drug detection limit.

5.6 Specificity Test
5.6.1

For each reagent in the kit other than those listed in appendix A, determine the fmal
color, if any, when mixed with each substance listed in table 4.
5.6.2

Determine whether the drugs listed in accordance with 4.1.1 can be differentiated from
each other and from the other substances listed in table 4 by the use of one or more of the.
reagents in the kit. To make this determination, use the information given in tables 1 and 4
for reagents listed in appendix A and the data obtained in accordance with 4.6.2 and 5.6.1
for reagents not listed in appendix A, or drug/reagent combinations not listed in table 1.

6

 APPENDIX A-REAGENTS
Use reagent grade chemicals.
A.I Cobalt (II) Thiocyanate
Dissolve 2.0 grams (g) of cobalt

an thiocyanate in 100 milliliters (ml) of distilled water.

A.2 Dillf!-Koppanyi Reagent, Modified

(In

Solution A. Dissolve 0.1 g of cobalt
acetate dihydrate in 100 ml of methanol. Add
0.2 ml of glacial acetic acid and mix.
Solution B. Add 5 ml of isopropylamine to 95 ml of methanol.
Procedure. Add 2 volumes of solution A to the drug, followed by 1 volume of solution
B.

A.3 Duquenois-Levine Reagent, Modified
Solution A. Add 2.5 ml of acetaldehyde and 2.0 g of vanillin to 100 ml of 95% ethanol.
Solution B. Concentrated Hydrochloric Acid.
Solution C. Chloroform.
Procedure. Add 1 volume of solution A to the drug and shake for 1 minute. Then add
solution B. Agitate gently, and determine the color produced. Add 3 volumes of solution C
and note whether the color is extracted from the mixture of A and B.

A.4 Mandelin Reagent
Dissolve 1.0 g of ammonium vanadate in 100 ml of concentrated sulfuric acid.

A.S Marquis Reagent
Carefully add 100 ml of concentrated sulfuric acid to 5 nil of 40% formaldehyde (v : v,
formaldehyde: water).

A.6 Nitric Acid
Concentrated.

A.7 Para-Dimethylaminobenzaldehyde (p-DMAB)
Add 2.0 g of p-DMAB to 50 ml of 95% ethanol and 50 ml of concentrated
acid.

A.S Ferric Chloride
Dissolve 2.0 g of anhydrous ferric chloride or 3.3 g of ferric chloride hexa-hydrate in
100 ml of distilled water.

A.9 Froehde Reagent
Dissolve 0.5 g of molybdic acid or sodium molybatc in 100 ml of hot concentrated
sulfuric acid.
7

 A.IO Mecke Reagent
Dissolve 1.0 g of selenious acid in 100 ml of concentrated sulfuric acid.
A.ll Zwikker Reagent
Solution A. Dissolve 0.5 g of copper (II) sulfate pentahydrate in 100 ml of distilled
water.
Solution B. Add 5 ml of pyridine to 95 ml of chloroform.
Procedure. Add 1 volume of solution A to the drug, followed by 1 volume of solution
B.

TABLE 1. Final colors produced by reagents A.I through A.ll with various drugs and
other substances. (+) means "and/or." Color. abbreviations are given at end of table.
Material

Reagent

Benzphetamine
Brompheniramine
Chlorpromazine . HCI
Cocaine . HCI
Darvon (propoxyphene . HCI)
Demerol . HCI
Doxepin . HCI
Heroin' HCI
Librium
Marezine (cyclizine· HCI)
Methadon . HCI
Methapyrilene . HCI
Opium
Phencyclidine
Procaine . HCI
Quinine, Quinine SaIts
Ritalin
Contac
Pentobarbital
Phenobarbital
Secobarbital
Tea
Mace

A.I
A.I
A.I
A.I*
A.I
A.l
A.I
A.I
A.I
A.I
A.I*
A.I
A.I
A.I
A.I*
A.I
A.I
A.2
A.2*
A.2*
A.2*
A.2
A.3

Marijuana

A.3*

Nutmeg

A.3

Tea
Aspirin
Benzphetamine
Brompheniramine
Chlorpromazine . HCI
Cocaine . HCI
Codeine Sulfate
Contac
D-Amphetamine . HCI
D-Methamphetamine . HCI
Darvon (propoxyphene . HCl)
Doxepin . HCI
Dristan

A.3
AA
AA*
AA
AA
AA*
AA*
AA
AA*
AA*
AA
AA
AA

8

Final Color (lSCC-NBS)
169.s.gB
16S. brill. g B + 177 brill. B
16S. brill. g B
169. s. g B + 17S. s. B
169. s. g B
169. s. g B
169. s. g B
169.s.gB
IS!. 1. B
17!.v.l.gB
lSI. 1. B
169. s. g B
146. d. G
16S. brill. g B
169. s. g B + 17S. s. B
17S. s. B
16S. brill. g B
31. p. Y Pk
21S. s. P
21S. s. P
21S. s. P
29. m. Y Pk
237. s. r P'
237. s. r P"
221. v. 1. P'
197. deep p B'
IS6. gy. B"
220. v. deep P'
244. p. r P'
244. p. r P"
226. v. p. P'
243. v. d. r p5
113.01Gy
119.1. YG
50. brill. 0
107. m. 01 + 13. deep R
51. deep 0
107. m. 01
S4. s. Y
164. m. bG
136. m. y G
44. d. r Br
21. blackish R
127. gy. 01 G

 Material
Excedrin .
Heroin' HCI
L-Isomethadon· HCI
Mace
MDA . S04 (3,4-methylenedioxyamphetamine)
Mescaline Sulfate
Methadon . RCI
Methapyrilene . HCI
Methaqualone
Methyprylon
Morphine
Opium
Oxycodone ·HCI
Procaine . HCI
Quinine
Ritalin
Salt, iodized
STP . HCI (2,S-dimethoxy-4methylamphetarnine)
TMA· HCI (trimethoxyamphetarnine)
Aspirin
Benzphetarnine
Chlorpromazine . HCI
Codeine Sulfate
D-Amphetamine
D-Methamphetamine . HCI
Darvon (propoxyphene . HC)
Demerol . HCI
Doxepin . HCI
Dristan
Excedrin
Heroin' HCI
LSD Tartrate
Mace
Marezine (cyclizine . HCI)
MDA . S04 (3,4-methylenedioxyamphetamine)
Mescaline Sulfate
Methadon . HCI
Methapyrilene . HCI
Morphine
Opium
Oxycodone . HCI
Pentabarbital
Phencyclidene
Phenobarbital
Ritalin
Secobarbital
STP.. HCI (2,5-dimethoxy-4methylamphetarnine)
Sugar
TMA . HCI (trimethoxyamphetamine)
Chlorpromazine' HCI
Codeine Sulfate
Doxepin . HCI
Excedrin
Heroin' HCI
LSD Tartrate
Mace
MDA . S04 (3,4-methylenedioxyamphetamine)

Reagent

Final Color (ISCC-NBS)

A.4
A.4*
A.4
A.4
A.4

108. d. 01
43. m. r Br
243. v. d. r P
46. gy. rBr
235. p Black

A.4*
A.4
A.4
A.4
A.4
A.4*
A.4*
A.4
A.4
A.4
A.4
A.4
A.4*

65. br Black
183. d. B
243. v. d. r P + 260.
35. s. RO
184. v. p. B
47. d. gy. r Br
94. I. 01 Br
68. s. 0 Y
51. deep 0
108. d. 01
68. s. 0 Y
51. deep 0
117. s. YO

A.4*
A.5

94. L 01 Br
12. s. R

A.S*
A.S
A.S*
A.S*
A.S*
A.5
A.5
A.S
A.5
A.5
A.S*
A.5
A.S
A.S
A.S*

41. deep r Br
260. v. d. pR
212. d. V
44. d. r Br + 34. v. r 0
44. d. r Br + 34. v. r 0
230. blackish P
56. deep Br
21. blackish R
241. m. r P
15. m. R
239. v. deep r P
235. p Black
244. p. r P
98. brill. g Y
267. Black

A.S*

A.S*

36. deep r 0
28. I. Y Pk
260. v. d. p R6
243. v. d. rP
44. d. rBr
201. d. p Br
78. d. Y Br
7.p.P
78. d. y Br
71. m. 0 Y
78. d. Y Br
101.I.gY

A.S
A.S*
A.6
A.6*
A.6
A.6
A.6*
A.6
A.6
A.6

46. gy. rBr
36. deep rO
101.1. gY
lOLl. gY
84. s. Y
68. s. 0 Y
89. p. Y
54. brO
40. s. r Br
101. I. gY

A.5
A.S
A.S*
A.S*
A.S*

A,S
A.5
A.S
A.S

A.S

9

v. d. p R

 Material
Mescaline Sulfate
Methapyrilene . HCI
Morphine
Opium
Oxycodone . HCI
STP . HCl (2,5-dimethoxy-4methyl amphetamine)
TMA . HCI (trimethoxyamphetamine)
LSD Tartrate
Baking Soda
Excedrin
Morphine
Aspirin
Chlorpromazine . HCI
Codeine Sulfate
Darvon (propoxyphene . HCI)
Doxepin . HCI
Dristan
Excedrin
Heroin' HCI
LSD Tartrate
Mace
Marezine (cyclizine . HCI)
MDA . SO. (3,4-methylenedioxyamphetamine)
Mescaline Sulfate
Methapyrilene . HC!
Morphine
Opium
Oxycodone . HC!
Phencyclidine
STP . HCI (2,5-dimethoxy-4methylamphetamine)
TMA . HCI (trimethoxyamphetamine)
Benzphetamine
Chlorpromazine . HCI
Codeine Sulfate
Darvon (propoxyphene . HCI)
Doxepin . HCI
Dristan
Excedrin
Heroin' HCI
LSD Tartrate
L-Isomethadon . HCI
Mace
Marezine (cyclizine . HCI)
MDA . SO. (3,4-methylenedioxyamphetamine)
Mescaline Sulfate
Methapyrilene . HCI
Morphine
Nutmeg
Opium
Oxycodone . HCI
Phencyclidine
STP . HCI (2,5-dimethoxy-4methyl amphetamine)
TMA . HCI (trimethoxyamphetamine)
Baking Soda
Excedrin
Mace
Pentobarbital

Reagent

Final Color (ISCC-NBS)

A.6*
A.6
A.6*
A.6*
A.6
A.6

41. deep r Br
44. d. rBr
67. brill. 0 Y
101.I.gY
86.1. Y
89. p. Y

A.6
A.7*
A.8
A.8
A.8*
A.9
A.9
A.9*
A.9
A.9
A.9
A.9
A.9*
A.9
A.9
A.9
A.9*

14. v. deep R
219. deep P
43. m. r Br
260. v. d. p R
67. brill. 0 Y
228. gy. P
21. blackish R
147. v. d. 0
230. blackish P
41. deep; Br
163.1. b 0
148. v. p 0
256. deep p R
138. v. d. yO
257. v. deep p R
10)'l.gY
157. g Black

A.9*
A.9
A.9*
A.9*
A.9
A.9
A.9*

84. s. Y
65. br Black
67. brill. 0 Y
96. d. OJ Br
84. s. Y
7.p.P
117. s. YO

A.9*
A.10*
A.IO
A.lO*
A.IO
A.IO
A.IO
A.lO
A.IO*
A.lO
A.lO
A.IO
A.lO
A.10*

183. d. Blue
101.1. gY
111. d. gy. 01 + 108. d. 01
175.v.d.gB
41. deep r Br
21. blackish R
94.1. 01 Br
93. y Oy
161. deep b 0
152. blackish 0
7. p. Pk
III. d. gy. 01
98. brill. g Y
183. d. B

A.I0*
A.IO
A.IO*
A.lO
A.IO*
A.lO
A.10
A.IO*

114. OJ Black
230. blackish P
166. v. d. b 0
65. br Black
114. 01 Black
107. m.OI
7.p.P
118. deep YO

A.lO*
A.11
A.11
A.11
A.ll*

75. deep y Br
181. I. B
144.1.0
120. m. YO
222.1. P

10

 Material

Final Color (ISCC-NBS)

Reagent
A.ll*
A.Il*
A.l1
A.II

Phenobarbital
Secobarbital
Tea
Tobacco
• Usual kit reagent for that particular drug.
, Color abbreviations used:
B = blue
b = bluish
Br= brown
br = brownish
brill. = brilliant
d. = dark
(] = green

g = greenish
Gy = gray

222.1. P
222.1. P
120. m. YG
136. m. y G

BY. = grayish
I. = light
m, = moderate
med. = medium
0= orange
01= olive
P = purple
p = purplish
p. = pale
Pk = pink

pk= pinkish
R= red
r= reddish
s. = strong
V = violet
v. = very or vivid
Y=yellow
y = yellowish

, Aqueous phase.
, Aqueous phase after chloroform extraction •
• Chloroform phase (mariiuana extraction usually rapid con pared to other materials).
, Not extracted into chloroform.
, Precipitate.

TABLE 2.

Reagent

I

Drug Detection Limits and Solvents for Sensitivity Test

Drug

Drug Detection
Lim!t, J.Lg

Solvent

60.0
15.0

CHCla
CHCl a
CHCla
CHCJ1
CHCla
CHCI:)

A.I
A.I

Cocaine . HCI
Methadon . HCI

A.2
A.2
A.2
A.2

Amobarbital
Pentobarbital
Phenobarbital
Secobarbital

100.0
100.0
100.0
100.0

A.3

Marijuana

350

AA
AA
AA
AA
AA

Codeine Sulfate
D-Amphetamine . HCI
Heroin' HCI
D-Methamphetamine . Hel
Morphine

5.0
10.0
20.0
150.0
5.0

H 2O
CHCI:)
CHCl a
CHCI:)
CHCla

A.5
A.5
A.5
A.5
A.5
A.S
A.5
A.S

Codeine Sulfate
D-Amphetamine . HCI
Heroin' HCI
LSD Tartrate
Mescaline Sulfate
Methadon . HCI
d-Methamphetamine . HCI
Morphine

1.0
10.0
10.0
5.0
1.0
1.0
5.0
5.0

H 2O
CHCI3
CHCI:)
H 2O
H 2O
CHCl a
CHCl a
CHCla

A.6

Mescaline Sulfate

1.0

H 2O

A.7

LSD Tartrate

5.C

H 2O

This quantity of marijuana assumes 0.5% active ingredients.

11

I

 TABLE 3.

Maximum Acceptable Drug Detection Limits for Reagents other than A.I
th!"ough A.7
Drug
Cocaine . HCI
Codeine Slllfate
D-Amphetamine . HCI
Heroin' HCi
LSD Tartrate
Marijuana
Mescaline Sulfate
Methadon . HCI
d-Methamphetamine . HCI
Morphine
Phenobarbital
I

600

50
100

200
50
1000

1

1(l

150
500
50
1000

This quantity of marijuana assumes 0.5% active ingredients.

TABLE 4. Substances to be Checked in Specificity Test.
( +) Indicates that a Color Reaction Occurs 1
Reagent
A.I
Baking Soda
Brompheniramine
Chlorpromazine . HCI
Contac
Darvon (propoxyphene . HCI)
Demerol . HCI
Doxepin . HCI
Dristan
Excedrin
Librium
Mace'
Marezin..· (cyclizine . HCI)
. HCI
Methaqualone
Methyprylon
Nutmeg'
Phencyclidine
Quinine Sulfate
Ritalin
Salt (Iodized)
Sugar
Tea, Cut Green'
Tobacco
1

+
+
+
+
+

A.2

A.3

+

A.4

A.5

+

+

+
+
+
+

+

+
+
+

+

+

+
+

+
+
+
+

+

+
+
+

+
+
+
+

+

+
+
+
+
+
+
+
+

A.6

A.7

A.S

A.9

+
+

+
+

+

+
+

+

A.IO A.II

+

+

+

+

+

+

+
+
+

+
+
+

+
+
+

+
+
+

+

+
+

+

+

+
+
+
+

Substances that gave no colors with these reagents are! D-galactose, glucose, mannitol, oregano, rosemary and thyme .

• Tea, mace and nutmeg interfere with Duquenois test, but not the Duquenois·Leviue modified test (A.3).

* U.S. GOVERNMENT PRINTING OFFICE: 19790-275-857
12

  

 

 

 

 

 

 

 

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