HPAI Biosecurity

Biosecure Entry Procedure
Information for Poultry and Egg Producers
Personnel should use a Biosecure Entry Procedure to cross the Line of Separation (LOS) which is established to
isolate poultry from possible sources of infection with avian influenza virus. The LOS consists of the building
walls separating the birds from the outside plus a marked line in the entry room to the poultry house/barn. The
following components are essential for a Biosecure Entry Procedure.

 Entry Area
 Establish a single, clearly marked entry area to
the building that excludes birds
 Visitors permitted entry to the bird housing
areas should fill out a visitor logbook
 Should include a clearly marked line within the
entry; this line is part of the LOS which
personnel cross from the outside to the bird side
of LOS

 Personnel & Equipment

Source: CFSPH, ISU

 Before crossing the LOS
 Remove street shoes/boots
 Remove outer clothing (e.g., a coat) to allow changing into
site-specific coveralls
 Leave personal items, such as cell phones and jewelry,
outside the LOS
 All equipment that crosses the LOS must be cleaned and
disinfected

Source: Pam Zaabel, CFSPH, ISU

 After crossing the LOS, before contacting birds
 Clean hands using hand sanitizer or soap
 Put on site-specific coveralls and boots, taking care not to
contaminate this apparel in the process
 Follow the same procedure, in reverse, when crossing back to the
outside of the LOS

Source: Pam Zaabel, CFSPH, ISU

The Danish Entry System is an example of a Biosecure Entry Procedure that has been used successfully. For
more information, see the video “The Danish Entry Principle” at
https://www.youtube.com/watch?v=N4NNkd_Kfqw

http://poultrybiosecurity.org/
Updated: 14 September 2015