CENTRAL ANALYTICAL LABORATORY
Report No.

6967

Date ..... ~g~S~. A~ -L~8 .....
Subject:

IRDC 137-092 :

FC- 95/Monkey
Dept. Name
Reque sCot: _ _ .~.w-.. Lo~....
Request No.

A69507

Dated

~eport:
Reference 137-087 by IRDC was a 90 day subacute Rhesus monkey toxicity
study of FC-95.
Incorrect (too high) feeding levels were used and all
animals died within the first few days. The feeding levels were lowered
and the study started again as 137-092. -The serum and livers were each
individually submitted for analysis. Each group contained 4 .monkeys,
2 male and 2 female as follows:
Group
~

II
III
IV

Dosaqe Level
0
0.5 mg/kg/day
1.5 mgikg!d~y
4.5 mg/kg/day

Survival
4/4

4/4
4/4
0/4

SERUM ANALYSIS
Monkey

Dosage Level

FC-95 in Serum (ppm)~

Blank on Method

-

2

7355M

0

40

7358M

0

20

7368F

0

15

7463M
7466F
7462M
7500F

0.5 mg/kg/day
"

150
150

1.5 mg/k~day
"

250
275

© Our

newly developed pyrolysis method was used.
Precision is estimated to be ±i0 - 25%.

3M MN02343995

1181.0001

 ~.R.’#6967

- 2 -

August 8, 1978

The high levels of FC-95 in the control serum cannot be explained.
For example, 7355M was run on 2 different days with the same experimental
results. Perhaps a total fluorine should be run on this sample to
determine if this level of F is present. See Table below containing
liver results for further discussion (Footnote 2).
¯ LIVER ANALYSIS
Monkey

Dosage Level

7355M

0

3000

50

7368F

0

1500~

2~

7463M

0.5 mg/kg/day
"

7466F
7462M

1.5 mg/kg/day
"

7500F
7484M

4.5 mg/kg/day

7502F

"

FC-95 in Liver(m~)~

FC-95 in Liver(ppm)

7000

i00

8000

I00

45000

650

40000

600

40000

650

80000

i000

The livers were stored in the refrigerator several months prior to
analysis resulting in the separation of some liquid. The high values
and physical size necessitates taking only partial livers in which
case the same ratio (estimated visually) of solid to separated liquid
was taken for analysis. In addition, recovery of FC-95 was estimated
from experiments where FC-95 was added to a control liver.
~Because of a higher than expected value for the control, a 0.133 g
liver sample was analyzed for total fluorine. The fluorine value
calculated as FC-95 is equivalent to ii ppm FC-95 in the liver.
As time permits, more work should be done to verify these "high"
control levels.

~/ c:R.A. Prokop

236-3

3M MN02343996

1181.0002